Microfluidics and Metabolomics Reveal Symbiotic Bacterial-Fungal Interactions Between Mortierella elongata and Burkholderia Include Metabolite Exchange.
Identifieur interne : 000837 ( Main/Exploration ); précédent : 000836; suivant : 000838Microfluidics and Metabolomics Reveal Symbiotic Bacterial-Fungal Interactions Between Mortierella elongata and Burkholderia Include Metabolite Exchange.
Auteurs : Jessie K. Uehling [États-Unis] ; Matthew R. Entler [États-Unis] ; Hannah R. Meredith [États-Unis] ; Larry J. Millet [États-Unis] ; Collin M. Timm [États-Unis] ; Jayde A. Aufrecht [États-Unis] ; Gregory M. Bonito [États-Unis] ; Nancy L. Engle [États-Unis] ; Jessy L. Labbé [États-Unis] ; Mitchel J. Doktycz [États-Unis] ; Scott T. Retterer [États-Unis] ; Joseph W. Spatafora [États-Unis] ; Jason E. Stajich [États-Unis] ; Timothy J. Tschaplinski [États-Unis] ; Rytas J. Vilgalys [États-Unis]Source :
- Frontiers in microbiology [ 1664-302X ] ; 2019.
Abstract
We identified two poplar (Populus sp.)-associated microbes, the fungus, Mortierella elongata strain AG77, and the bacterium, Burkholderia strain BT03, that mutually promote each other's growth. Using culture assays in concert with a novel microfluidic device to generate time-lapse videos, we found growth specific media differing in pH and pre-conditioned by microbial growth led to increased fungal and bacterial growth rates. Coupling microfluidics and comparative metabolomics data results indicated that observed microbial growth stimulation involves metabolic exchange during two ordered events. The first is an emission of fungal metabolites, including organic acids used or modified by bacteria. A second signal of unknown nature is produced by bacteria which increases fungal growth rates. We find this symbiosis is initiated in part by metabolic exchange involving fungal organic acids.
DOI: 10.3389/fmicb.2019.02163
PubMed: 31632357
PubMed Central: PMC6779839
Affiliations:
- États-Unis
- Californie, Caroline du Nord, Maryland, Michigan, Oregon, Tennessee
- East Lansing
- Université d'État du Michigan
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Uehling</name><affiliation wicri:level="2"><nlm:affiliation>Department of Botany and Plant Pathology, Oregon State University, Corvallis, OR, United States.</nlm:affiliation><country xml:lang="fr">États-Unis</country><wicri:regionArea>Department of Botany and Plant Pathology, Oregon State University, Corvallis, OR</wicri:regionArea><placeName><region type="state">Oregon</region></placeName></affiliation><affiliation wicri:level="2"><nlm:affiliation>Department of Biology, Duke University, Durham, NC, United States.</nlm:affiliation><country xml:lang="fr">États-Unis</country><wicri:regionArea>Department of Biology, Duke University, Durham, NC</wicri:regionArea><placeName><region type="state">Caroline du Nord</region></placeName></affiliation></author><author><name sortKey="Entler, Matthew R" sort="Entler, Matthew R" uniqKey="Entler M" first="Matthew R" last="Entler">Matthew R. Entler</name><affiliation wicri:level="2"><nlm:affiliation>Biosciences Division, Oak Ridge National Laboratory, Oak Ridge, TN, United States.</nlm:affiliation><country xml:lang="fr">États-Unis</country><wicri:regionArea>Biosciences Division, Oak Ridge National Laboratory, Oak Ridge, TN</wicri:regionArea><placeName><region type="state">Tennessee</region></placeName></affiliation></author><author><name sortKey="Meredith, Hannah R" sort="Meredith, Hannah R" uniqKey="Meredith H" first="Hannah R" last="Meredith">Hannah R. 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Timm</name><affiliation wicri:level="2"><nlm:affiliation>Biosciences Division, Oak Ridge National Laboratory, Oak Ridge, TN, United States.</nlm:affiliation><country xml:lang="fr">États-Unis</country><wicri:regionArea>Biosciences Division, Oak Ridge National Laboratory, Oak Ridge, TN</wicri:regionArea><placeName><region type="state">Tennessee</region></placeName></affiliation></author><author><name sortKey="Aufrecht, Jayde A" sort="Aufrecht, Jayde A" uniqKey="Aufrecht J" first="Jayde A" last="Aufrecht">Jayde A. 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Bonito</name><affiliation wicri:level="4"><nlm:affiliation>Department of Plant, Soil and Microbial Sciences, Michigan State University, East Lansing, MI, United States.</nlm:affiliation><country xml:lang="fr">États-Unis</country><wicri:regionArea>Department of Plant, Soil and Microbial Sciences, Michigan State University, East Lansing, MI</wicri:regionArea><placeName><region type="state">Michigan</region><settlement type="city">East Lansing</settlement></placeName><orgName type="university">Université d'État du Michigan</orgName></affiliation></author><author><name sortKey="Engle, Nancy L" sort="Engle, Nancy L" uniqKey="Engle N" first="Nancy L" last="Engle">Nancy L. 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Doktycz</name><affiliation wicri:level="2"><nlm:affiliation>Biosciences Division, Oak Ridge National Laboratory, Oak Ridge, TN, United States.</nlm:affiliation><country xml:lang="fr">États-Unis</country><wicri:regionArea>Biosciences Division, Oak Ridge National Laboratory, Oak Ridge, TN</wicri:regionArea><placeName><region type="state">Tennessee</region></placeName></affiliation><affiliation wicri:level="2"><nlm:affiliation>Genome Science & Technology, The University of Tennessee, Knoxville, Knoxville, TN, United States.</nlm:affiliation><country xml:lang="fr">États-Unis</country><wicri:regionArea>Genome Science & Technology, The University of Tennessee, Knoxville, Knoxville, TN</wicri:regionArea><placeName><region type="state">Tennessee</region></placeName></affiliation><affiliation wicri:level="2"><nlm:affiliation>Center for Nanophase Materials Sciences, Oak Ridge National Laboratory, Oak Ridge, TN, United States.</nlm:affiliation><country xml:lang="fr">États-Unis</country><wicri:regionArea>Center for Nanophase Materials Sciences, Oak Ridge National Laboratory, Oak Ridge, TN</wicri:regionArea><placeName><region type="state">Tennessee</region></placeName></affiliation></author><author><name sortKey="Retterer, Scott T" sort="Retterer, Scott T" uniqKey="Retterer S" first="Scott T" last="Retterer">Scott T. 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Spatafora</name><affiliation wicri:level="2"><nlm:affiliation>Department of Botany and Plant Pathology, Oregon State University, Corvallis, OR, United States.</nlm:affiliation><country xml:lang="fr">États-Unis</country><wicri:regionArea>Department of Botany and Plant Pathology, Oregon State University, Corvallis, OR</wicri:regionArea><placeName><region type="state">Oregon</region></placeName></affiliation></author><author><name sortKey="Stajich, Jason E" sort="Stajich, Jason E" uniqKey="Stajich J" first="Jason E" last="Stajich">Jason E. Stajich</name><affiliation wicri:level="2"><nlm:affiliation>Department of Microbiology and Plant Pathology, Institute for Integrative Genome Biology, University of California, Riverside, Riverside, CA, United States.</nlm:affiliation><country xml:lang="fr">États-Unis</country><wicri:regionArea>Department of Microbiology and Plant Pathology, Institute for Integrative Genome Biology, University of California, Riverside, Riverside, CA</wicri:regionArea><placeName><region type="state">Californie</region></placeName></affiliation></author><author><name sortKey="Tschaplinski, Timothy J" sort="Tschaplinski, Timothy J" uniqKey="Tschaplinski T" first="Timothy J" last="Tschaplinski">Timothy J. Tschaplinski</name><affiliation wicri:level="2"><nlm:affiliation>Biosciences Division, Oak Ridge National Laboratory, Oak Ridge, TN, United States.</nlm:affiliation><country xml:lang="fr">États-Unis</country><wicri:regionArea>Biosciences Division, Oak Ridge National Laboratory, Oak Ridge, TN</wicri:regionArea><placeName><region type="state">Tennessee</region></placeName></affiliation></author><author><name sortKey="Vilgalys, Rytas J" sort="Vilgalys, Rytas J" uniqKey="Vilgalys R" first="Rytas J" last="Vilgalys">Rytas J. Vilgalys</name><affiliation wicri:level="2"><nlm:affiliation>Department of Biology, Duke University, Durham, NC, United States.</nlm:affiliation><country xml:lang="fr">États-Unis</country><wicri:regionArea>Department of Biology, Duke University, Durham, NC</wicri:regionArea><placeName><region type="state">Caroline du Nord</region></placeName></affiliation></author></titleStmt><publicationStmt><idno type="wicri:source">PubMed</idno><date when="2019">2019</date><idno type="RBID">pubmed:31632357</idno><idno type="pmid">31632357</idno><idno type="doi">10.3389/fmicb.2019.02163</idno><idno type="pmc">PMC6779839</idno><idno type="wicri:Area/Main/Corpus">000657</idno><idno type="wicri:explorRef" wicri:stream="Main" wicri:step="Corpus" wicri:corpus="PubMed">000657</idno><idno type="wicri:Area/Main/Curation">000657</idno><idno type="wicri:explorRef" wicri:stream="Main" wicri:step="Curation">000657</idno><idno type="wicri:Area/Main/Exploration">000657</idno></publicationStmt><sourceDesc><biblStruct><analytic><title xml:lang="en">Microfluidics and Metabolomics Reveal Symbiotic Bacterial-Fungal Interactions Between <i>Mortierella elongata</i> and <i>Burkholderia</i> Include Metabolite Exchange.</title><author><name sortKey="Uehling, Jessie K" sort="Uehling, Jessie K" uniqKey="Uehling J" first="Jessie K" last="Uehling">Jessie K. Uehling</name><affiliation wicri:level="2"><nlm:affiliation>Department of Botany and Plant Pathology, Oregon State University, Corvallis, OR, United States.</nlm:affiliation><country xml:lang="fr">États-Unis</country><wicri:regionArea>Department of Botany and Plant Pathology, Oregon State University, Corvallis, OR</wicri:regionArea><placeName><region type="state">Oregon</region></placeName></affiliation><affiliation wicri:level="2"><nlm:affiliation>Department of Biology, Duke University, Durham, NC, United States.</nlm:affiliation><country xml:lang="fr">États-Unis</country><wicri:regionArea>Department of Biology, Duke University, Durham, NC</wicri:regionArea><placeName><region type="state">Caroline du Nord</region></placeName></affiliation></author><author><name sortKey="Entler, Matthew R" sort="Entler, Matthew R" uniqKey="Entler M" first="Matthew R" last="Entler">Matthew R. Entler</name><affiliation wicri:level="2"><nlm:affiliation>Biosciences Division, Oak Ridge National Laboratory, Oak Ridge, TN, United States.</nlm:affiliation><country xml:lang="fr">États-Unis</country><wicri:regionArea>Biosciences Division, Oak Ridge National Laboratory, Oak Ridge, TN</wicri:regionArea><placeName><region type="state">Tennessee</region></placeName></affiliation></author><author><name sortKey="Meredith, Hannah R" sort="Meredith, Hannah R" uniqKey="Meredith H" first="Hannah R" last="Meredith">Hannah R. Meredith</name><affiliation wicri:level="2"><nlm:affiliation>Department of Epidemiology, Johns Hopkins Bloomberg School of Public Health, Baltimore, MD, United States.</nlm:affiliation><country xml:lang="fr">États-Unis</country><wicri:regionArea>Department of Epidemiology, Johns Hopkins Bloomberg School of Public Health, Baltimore, MD</wicri:regionArea><placeName><region type="state">Maryland</region></placeName></affiliation><affiliation wicri:level="2"><nlm:affiliation>Department of Biomedical Engineering, Duke University, Durham, NC, United States.</nlm:affiliation><country xml:lang="fr">États-Unis</country><wicri:regionArea>Department of Biomedical Engineering, Duke University, Durham, NC</wicri:regionArea><placeName><region type="state">Caroline du Nord</region></placeName></affiliation></author><author><name sortKey="Millet, Larry J" sort="Millet, Larry J" uniqKey="Millet L" first="Larry J" last="Millet">Larry J. Millet</name><affiliation wicri:level="2"><nlm:affiliation>Biosciences Division, Oak Ridge National Laboratory, Oak Ridge, TN, United States.</nlm:affiliation><country xml:lang="fr">États-Unis</country><wicri:regionArea>Biosciences Division, Oak Ridge National Laboratory, Oak Ridge, TN</wicri:regionArea><placeName><region type="state">Tennessee</region></placeName></affiliation><affiliation wicri:level="2"><nlm:affiliation>The Bredesen Center, The University of Tennessee, Knoxville, Knoxville, TN, United States.</nlm:affiliation><country xml:lang="fr">États-Unis</country><wicri:regionArea>The Bredesen Center, The University of Tennessee, Knoxville, Knoxville, TN</wicri:regionArea><placeName><region type="state">Tennessee</region></placeName></affiliation></author><author><name sortKey="Timm, Collin M" sort="Timm, Collin M" uniqKey="Timm C" first="Collin M" last="Timm">Collin M. Timm</name><affiliation wicri:level="2"><nlm:affiliation>Biosciences Division, Oak Ridge National Laboratory, Oak Ridge, TN, United States.</nlm:affiliation><country xml:lang="fr">États-Unis</country><wicri:regionArea>Biosciences Division, Oak Ridge National Laboratory, Oak Ridge, TN</wicri:regionArea><placeName><region type="state">Tennessee</region></placeName></affiliation></author><author><name sortKey="Aufrecht, Jayde A" sort="Aufrecht, Jayde A" uniqKey="Aufrecht J" first="Jayde A" last="Aufrecht">Jayde A. Aufrecht</name><affiliation wicri:level="2"><nlm:affiliation>Biosciences Division, Oak Ridge National Laboratory, Oak Ridge, TN, United States.</nlm:affiliation><country xml:lang="fr">États-Unis</country><wicri:regionArea>Biosciences Division, Oak Ridge National Laboratory, Oak Ridge, TN</wicri:regionArea><placeName><region type="state">Tennessee</region></placeName></affiliation></author><author><name sortKey="Bonito, Gregory M" sort="Bonito, Gregory M" uniqKey="Bonito G" first="Gregory M" last="Bonito">Gregory M. Bonito</name><affiliation wicri:level="4"><nlm:affiliation>Department of Plant, Soil and Microbial Sciences, Michigan State University, East Lansing, MI, United States.</nlm:affiliation><country xml:lang="fr">États-Unis</country><wicri:regionArea>Department of Plant, Soil and Microbial Sciences, Michigan State University, East Lansing, MI</wicri:regionArea><placeName><region type="state">Michigan</region><settlement type="city">East Lansing</settlement></placeName><orgName type="university">Université d'État du Michigan</orgName></affiliation></author><author><name sortKey="Engle, Nancy L" sort="Engle, Nancy L" uniqKey="Engle N" first="Nancy L" last="Engle">Nancy L. Engle</name><affiliation wicri:level="2"><nlm:affiliation>Biosciences Division, Oak Ridge National Laboratory, Oak Ridge, TN, United States.</nlm:affiliation><country xml:lang="fr">États-Unis</country><wicri:regionArea>Biosciences Division, Oak Ridge National Laboratory, Oak Ridge, TN</wicri:regionArea><placeName><region type="state">Tennessee</region></placeName></affiliation></author><author><name sortKey="Labbe, Jessy L" sort="Labbe, Jessy L" uniqKey="Labbe J" first="Jessy L" last="Labbé">Jessy L. Labbé</name><affiliation wicri:level="2"><nlm:affiliation>Biosciences Division, Oak Ridge National Laboratory, Oak Ridge, TN, United States.</nlm:affiliation><country xml:lang="fr">États-Unis</country><wicri:regionArea>Biosciences Division, Oak Ridge National Laboratory, Oak Ridge, TN</wicri:regionArea><placeName><region type="state">Tennessee</region></placeName></affiliation><affiliation wicri:level="2"><nlm:affiliation>Genome Science & Technology, The University of Tennessee, Knoxville, Knoxville, TN, United States.</nlm:affiliation><country xml:lang="fr">États-Unis</country><wicri:regionArea>Genome Science & Technology, The University of Tennessee, Knoxville, Knoxville, TN</wicri:regionArea><placeName><region type="state">Tennessee</region></placeName></affiliation></author><author><name sortKey="Doktycz, Mitchel J" sort="Doktycz, Mitchel J" uniqKey="Doktycz M" first="Mitchel J" last="Doktycz">Mitchel J. Doktycz</name><affiliation wicri:level="2"><nlm:affiliation>Biosciences Division, Oak Ridge National Laboratory, Oak Ridge, TN, United States.</nlm:affiliation><country xml:lang="fr">États-Unis</country><wicri:regionArea>Biosciences Division, Oak Ridge National Laboratory, Oak Ridge, TN</wicri:regionArea><placeName><region type="state">Tennessee</region></placeName></affiliation><affiliation wicri:level="2"><nlm:affiliation>Genome Science & Technology, The University of Tennessee, Knoxville, Knoxville, TN, United States.</nlm:affiliation><country xml:lang="fr">États-Unis</country><wicri:regionArea>Genome Science & Technology, The University of Tennessee, Knoxville, Knoxville, TN</wicri:regionArea><placeName><region type="state">Tennessee</region></placeName></affiliation><affiliation wicri:level="2"><nlm:affiliation>Center for Nanophase Materials Sciences, Oak Ridge National Laboratory, Oak Ridge, TN, United States.</nlm:affiliation><country xml:lang="fr">États-Unis</country><wicri:regionArea>Center for Nanophase Materials Sciences, Oak Ridge National Laboratory, Oak Ridge, TN</wicri:regionArea><placeName><region type="state">Tennessee</region></placeName></affiliation></author><author><name sortKey="Retterer, Scott T" sort="Retterer, Scott T" uniqKey="Retterer S" first="Scott T" last="Retterer">Scott T. Retterer</name><affiliation wicri:level="2"><nlm:affiliation>Biosciences Division, Oak Ridge National Laboratory, Oak Ridge, TN, United States.</nlm:affiliation><country xml:lang="fr">États-Unis</country><wicri:regionArea>Biosciences Division, Oak Ridge National Laboratory, Oak Ridge, TN</wicri:regionArea><placeName><region type="state">Tennessee</region></placeName></affiliation><affiliation wicri:level="2"><nlm:affiliation>Center for Nanophase Materials Sciences, Oak Ridge National Laboratory, Oak Ridge, TN, United States.</nlm:affiliation><country xml:lang="fr">États-Unis</country><wicri:regionArea>Center for Nanophase Materials Sciences, Oak Ridge National Laboratory, Oak Ridge, TN</wicri:regionArea><placeName><region type="state">Tennessee</region></placeName></affiliation></author><author><name sortKey="Spatafora, Joseph W" sort="Spatafora, Joseph W" uniqKey="Spatafora J" first="Joseph W" last="Spatafora">Joseph W. Spatafora</name><affiliation wicri:level="2"><nlm:affiliation>Department of Botany and Plant Pathology, Oregon State University, Corvallis, OR, United States.</nlm:affiliation><country xml:lang="fr">États-Unis</country><wicri:regionArea>Department of Botany and Plant Pathology, Oregon State University, Corvallis, OR</wicri:regionArea><placeName><region type="state">Oregon</region></placeName></affiliation></author><author><name sortKey="Stajich, Jason E" sort="Stajich, Jason E" uniqKey="Stajich J" first="Jason E" last="Stajich">Jason E. Stajich</name><affiliation wicri:level="2"><nlm:affiliation>Department of Microbiology and Plant Pathology, Institute for Integrative Genome Biology, University of California, Riverside, Riverside, CA, United States.</nlm:affiliation><country xml:lang="fr">États-Unis</country><wicri:regionArea>Department of Microbiology and Plant Pathology, Institute for Integrative Genome Biology, University of California, Riverside, Riverside, CA</wicri:regionArea><placeName><region type="state">Californie</region></placeName></affiliation></author><author><name sortKey="Tschaplinski, Timothy J" sort="Tschaplinski, Timothy J" uniqKey="Tschaplinski T" first="Timothy J" last="Tschaplinski">Timothy J. Tschaplinski</name><affiliation wicri:level="2"><nlm:affiliation>Biosciences Division, Oak Ridge National Laboratory, Oak Ridge, TN, United States.</nlm:affiliation><country xml:lang="fr">États-Unis</country><wicri:regionArea>Biosciences Division, Oak Ridge National Laboratory, Oak Ridge, TN</wicri:regionArea><placeName><region type="state">Tennessee</region></placeName></affiliation></author><author><name sortKey="Vilgalys, Rytas J" sort="Vilgalys, Rytas J" uniqKey="Vilgalys R" first="Rytas J" last="Vilgalys">Rytas J. Vilgalys</name><affiliation wicri:level="2"><nlm:affiliation>Department of Biology, Duke University, Durham, NC, United States.</nlm:affiliation><country xml:lang="fr">États-Unis</country><wicri:regionArea>Department of Biology, Duke University, Durham, NC</wicri:regionArea><placeName><region type="state">Caroline du Nord</region></placeName></affiliation></author></analytic><series><title level="j">Frontiers in microbiology</title><idno type="ISSN">1664-302X</idno><imprint><date when="2019" type="published">2019</date></imprint></series></biblStruct></sourceDesc></fileDesc><profileDesc><textClass></textClass></profileDesc></teiHeader><front><div type="abstract" xml:lang="en">We identified two poplar (<i>Populus</i> sp.)-associated microbes, the fungus, <i>Mortierella elongata</i> strain AG77, and the bacterium, <i>Burkholderia</i> strain BT03, that mutually promote each other's growth. Using culture assays in concert with a novel microfluidic device to generate time-lapse videos, we found growth specific media differing in pH and pre-conditioned by microbial growth led to increased fungal and bacterial growth rates. Coupling microfluidics and comparative metabolomics data results indicated that observed microbial growth stimulation involves metabolic exchange during two ordered events. The first is an emission of fungal metabolites, including organic acids used or modified by bacteria. A second signal of unknown nature is produced by bacteria which increases fungal growth rates. We find this symbiosis is initiated in part by metabolic exchange involving fungal organic acids.</div></front></TEI><pubmed><MedlineCitation Status="PubMed-not-MEDLINE" Owner="NLM"><PMID Version="1">31632357</PMID><DateRevised><Year>2020</Year><Month>10</Month><Day>01</Day></DateRevised><Article PubModel="Electronic-eCollection"><Journal><ISSN IssnType="Print">1664-302X</ISSN><JournalIssue CitedMedium="Print"><Volume>10</Volume><PubDate><Year>2019</Year></PubDate></JournalIssue><Title>Frontiers in microbiology</Title><ISOAbbreviation>Front Microbiol</ISOAbbreviation></Journal><ArticleTitle>Microfluidics and Metabolomics Reveal Symbiotic Bacterial-Fungal Interactions Between <i>Mortierella elongata</i> and <i>Burkholderia</i> Include Metabolite Exchange.</ArticleTitle><Pagination><MedlinePgn>2163</MedlinePgn></Pagination><ELocationID EIdType="doi" ValidYN="Y">10.3389/fmicb.2019.02163</ELocationID><Abstract><AbstractText>We identified two poplar (<i>Populus</i> sp.)-associated microbes, the fungus, <i>Mortierella elongata</i> strain AG77, and the bacterium, <i>Burkholderia</i> strain BT03, that mutually promote each other's growth. Using culture assays in concert with a novel microfluidic device to generate time-lapse videos, we found growth specific media differing in pH and pre-conditioned by microbial growth led to increased fungal and bacterial growth rates. Coupling microfluidics and comparative metabolomics data results indicated that observed microbial growth stimulation involves metabolic exchange during two ordered events. The first is an emission of fungal metabolites, including organic acids used or modified by bacteria. A second signal of unknown nature is produced by bacteria which increases fungal growth rates. We find this symbiosis is initiated in part by metabolic exchange involving fungal organic acids.</AbstractText><CopyrightInformation>Copyright © 2019 Uehling, Entler, Meredith, Millet, Timm, Aufrecht, Bonito, Engle, Labbé, Doktycz, Retterer, Spatafora, Stajich, Tschaplinski and Vilgalys.</CopyrightInformation></Abstract><AuthorList CompleteYN="Y"><Author ValidYN="Y"><LastName>Uehling</LastName><ForeName>Jessie K</ForeName><Initials>JK</Initials><AffiliationInfo><Affiliation>Department of Botany and Plant Pathology, Oregon State University, Corvallis, OR, United States.</Affiliation></AffiliationInfo><AffiliationInfo><Affiliation>Department of Biology, Duke University, Durham, NC, United States.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Entler</LastName><ForeName>Matthew R</ForeName><Initials>MR</Initials><AffiliationInfo><Affiliation>Biosciences Division, Oak Ridge National Laboratory, Oak Ridge, TN, United States.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Meredith</LastName><ForeName>Hannah R</ForeName><Initials>HR</Initials><AffiliationInfo><Affiliation>Department of Epidemiology, Johns Hopkins Bloomberg School of Public Health, Baltimore, MD, United States.</Affiliation></AffiliationInfo><AffiliationInfo><Affiliation>Department of Biomedical Engineering, Duke University, Durham, NC, United States.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Millet</LastName><ForeName>Larry J</ForeName><Initials>LJ</Initials><AffiliationInfo><Affiliation>Biosciences Division, Oak Ridge National Laboratory, Oak Ridge, TN, United States.</Affiliation></AffiliationInfo><AffiliationInfo><Affiliation>The Bredesen Center, The University of Tennessee, Knoxville, Knoxville, TN, United States.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Timm</LastName><ForeName>Collin M</ForeName><Initials>CM</Initials><AffiliationInfo><Affiliation>Biosciences Division, Oak Ridge National Laboratory, Oak Ridge, TN, United States.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Aufrecht</LastName><ForeName>Jayde A</ForeName><Initials>JA</Initials><AffiliationInfo><Affiliation>Biosciences Division, Oak Ridge National Laboratory, Oak Ridge, TN, United States.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Bonito</LastName><ForeName>Gregory M</ForeName><Initials>GM</Initials><AffiliationInfo><Affiliation>Department of Plant, Soil and Microbial Sciences, Michigan State University, East Lansing, MI, United States.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Engle</LastName><ForeName>Nancy L</ForeName><Initials>NL</Initials><AffiliationInfo><Affiliation>Biosciences Division, Oak Ridge National Laboratory, Oak Ridge, TN, United States.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Labbé</LastName><ForeName>Jessy L</ForeName><Initials>JL</Initials><AffiliationInfo><Affiliation>Biosciences Division, Oak Ridge National Laboratory, Oak Ridge, TN, United States.</Affiliation></AffiliationInfo><AffiliationInfo><Affiliation>Genome Science & Technology, The University of Tennessee, Knoxville, Knoxville, TN, United States.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Doktycz</LastName><ForeName>Mitchel J</ForeName><Initials>MJ</Initials><AffiliationInfo><Affiliation>Biosciences Division, Oak Ridge National Laboratory, Oak Ridge, TN, United States.</Affiliation></AffiliationInfo><AffiliationInfo><Affiliation>Genome Science & Technology, The University of Tennessee, Knoxville, Knoxville, TN, United States.</Affiliation></AffiliationInfo><AffiliationInfo><Affiliation>Center for Nanophase Materials Sciences, Oak Ridge National Laboratory, Oak Ridge, TN, United States.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Retterer</LastName><ForeName>Scott T</ForeName><Initials>ST</Initials><AffiliationInfo><Affiliation>Biosciences Division, Oak Ridge National Laboratory, Oak Ridge, TN, United States.</Affiliation></AffiliationInfo><AffiliationInfo><Affiliation>Center for Nanophase Materials Sciences, Oak Ridge National Laboratory, Oak Ridge, TN, United States.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Spatafora</LastName><ForeName>Joseph W</ForeName><Initials>JW</Initials><AffiliationInfo><Affiliation>Department of Botany and Plant Pathology, Oregon State University, Corvallis, OR, United States.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Stajich</LastName><ForeName>Jason E</ForeName><Initials>JE</Initials><AffiliationInfo><Affiliation>Department of Microbiology and Plant Pathology, Institute for Integrative Genome Biology, University of California, Riverside, Riverside, CA, United States.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Tschaplinski</LastName><ForeName>Timothy J</ForeName><Initials>TJ</Initials><AffiliationInfo><Affiliation>Biosciences Division, Oak Ridge National Laboratory, Oak Ridge, TN, United States.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Vilgalys</LastName><ForeName>Rytas J</ForeName><Initials>RJ</Initials><AffiliationInfo><Affiliation>Department of Biology, Duke University, Durham, NC, United States.</Affiliation></AffiliationInfo></Author></AuthorList><Language>eng</Language><PublicationTypeList><PublicationType UI="D016428">Journal Article</PublicationType></PublicationTypeList><ArticleDate DateType="Electronic"><Year>2019</Year><Month>10</Month><Day>01</Day></ArticleDate></Article><MedlineJournalInfo><Country>Switzerland</Country><MedlineTA>Front Microbiol</MedlineTA><NlmUniqueID>101548977</NlmUniqueID><ISSNLinking>1664-302X</ISSNLinking></MedlineJournalInfo><KeywordList Owner="NOTNLM"><Keyword MajorTopicYN="N">Burkholderia</Keyword><Keyword MajorTopicYN="N">Mortierella elongata</Keyword><Keyword MajorTopicYN="N">bacterial–fungal interactions</Keyword><Keyword MajorTopicYN="N">fatty acid</Keyword><Keyword MajorTopicYN="N">metabolomics</Keyword><Keyword MajorTopicYN="N">microfluidics</Keyword><Keyword MajorTopicYN="N">plant associated microbes</Keyword><Keyword MajorTopicYN="N">symbiotic evolution</Keyword></KeywordList></MedlineCitation><PubmedData><History><PubMedPubDate PubStatus="received"><Year>2019</Year><Month>05</Month><Day>10</Day></PubMedPubDate><PubMedPubDate PubStatus="accepted"><Year>2019</Year><Month>09</Month><Day>03</Day></PubMedPubDate><PubMedPubDate PubStatus="entrez"><Year>2019</Year><Month>10</Month><Day>22</Day><Hour>6</Hour><Minute>0</Minute></PubMedPubDate><PubMedPubDate PubStatus="pubmed"><Year>2019</Year><Month>10</Month><Day>22</Day><Hour>6</Hour><Minute>0</Minute></PubMedPubDate><PubMedPubDate 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